Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (5): 272-275.doi: 10.3969/j.issn.1004-616x.2005.05.005

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Effects of Different Stressful Factors on HeLa Cell roliferation，Apoptosis and HSP70 Expression

LI Lian;Song Wei-long;Zhou Lei;Zhang Zhong-xia;WANG Gen-lin   

  1. College of Animal Science and Technology, Nanjing Agriculture University
  • Received:2005-01-01 Revised:2005-05-31 Online:2005-09-30 Published:2005-09-30

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Abstract: BACKGROUND ＆ AIM: To investigate the effects of different stress factors on HeLa cell proliferation,apoptosis and heat shock protein 70(HSP70) expression. MATERIAL AND METHODS:Cell culture technique,3_(4,5_dimethylthiazol_2_yl)_2,5_diphenyl tetrazolium(MTT) flow cytometry,immunocytochemistry,western dot. RESULTS:The present study showed that cell apoptosis appeared in all experiment group;there was a tendence to increase HSP70 expression treated with Ethylenediamine tetra_acetic acid (EDTA) and Ca2＋; The protein level of HSP70 was elevated significantly in the groups of H2O2,heating and aminophylline (P<0.01) than control group; the protein level of HSP70 was higher (P<0.05) than control group in the groups of dexamthasone and tween_80. CONCLUSION: The results suggested that the expression of HSP70 in HeLa cells could be elevated and apoptosis could be induced by stress factors.

Key words: HeLa cell, cell proliferation, apoptosis, heat shock protein 70